NIST8671

NISTmAb, Humanized IgG1_k Monoclonal Antibody

NIST^® SRM^® 8671

• UNSPSC Code: 41116107
• NACRES: NA.41

Properties

• antibody product type: primary antibodies
• grade: certified reference material
• form: liquid
• manufacturer/tradename: NIST^®
• technique(s): UV/Vis spectroscopy: suitable; liquid chromatography (LC): suitable; mass spectrometry (MS): suitable
• format: matrix material

Description

General description

NISTmAb, Humanized IgG1k Monoclonal Antibody is a recombinant antibody expressed in murine suspension culture. It is a homodimer with two identical light chains and two identical heavy chains linked through inter- and intra- chain disulfide bonds with a molecular weight of 150 kDa. The protein possesses a low abundance of post-translational modifications including methionine oxidation, deamidation, and glycation. This humanized monoclonal antibody also has N-terminal pyroglutamination, C-terminal lysine clipping, and glycosylation of the heavy chains.

A unit of RM 8671 consists of one internal-threaded polypropylene cryovial containing 800 µL of 10 mg/mL NIST IgG1κ monoclonal antibody (NISTmAb) in 12.5 mmol/L L-histidine, 12.5 mmol/L L-histidine HCl (pH 6.0).

SRM 8671_Cert

SRM 8671_SDS

Application

NISTmAb, Humanized IgG1_k Monoclonal Antibody has been used as a standard for testing de novo sequencing algorithms on bottom-up mass spectrometry.This reference material (RM) is primarily intended for evaluating the performance of methods for determining physiochemical and biophysical attributes of monoclonal antibodies. It also provides a representative test molecule for developing novel technology in therapeutic protein characterization. Other potential uses include:

• system suitability tests
• establishing method or instrument performance and variability
• comparing changing analytical test methods
• assisting in method qualification

Analysis Note

NIST8671 is intended for research use. It is not intended for animal or human consumption, clinical testing or therapeutic use.

Other Notes

• RM 8671 is intended for research use.
• Details on expiration, storage, safety, usage, and source are provided in the NIST certificate. 
• Information on biomaterials, disposal, and transport is available in the SDS.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology

SRM is a registered trademark of National Institute of Standards and Technology

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable