318752

Bromothymol Blue sodium salt solution

0.04 wt. % in H₂O

• Empirical Formula (Hill Notation): C₂₇H₂₇Br₂NaO₅S
• CAS Number: 34722-90-2
• Molecular Weight: 646.36
• MDL number: MFCD00077263
• UNSPSC Code: 12171500
• PubChem Substance ID: 24859144
• NACRES: NA.47

Properties

• form: liquid
• Quality Level: 200
• concentration: 0.04 wt. % in H₂O
• pH: 6.0-7.6, yellow to blue
• density: 0.99 g/mL at 25 °C (lit.)
• λmax: 615 nm
• ε (extinction coefficient): ≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L; ≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
• InChI: 1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
• InChI key: NMKFVGALBGZKGW-FKWCIMQXSA-M

Description

General description

Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Application

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.

Biochem/physiol Actions

Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves