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18189

Supelco

2,4-Dinitrophenylhydrazine hydrochloric acid solution

~0.005 M in ethanol, for TLC derivatization

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About This Item

Empirical Formula (Hill Notation):
C6H6N4O4
CAS Number:
119-26-6
Molecular Weight:
198.14
Beilstein:
615586
MDL number:
MFCD00007578
UNSPSC Code:
41116105
PubChem Substance ID:
329751648
NACRES:
NA.22
grade:
for TLC derivatization
technique(s):
thin layer chromatography (TLC): suitable
Pricing and availability is not currently available.

grade

for TLC derivatization

Quality Level

100

concentration

~0.005 M in ethanol

technique(s)

thin layer chromatography (TLC): suitable

refractive index

n20/D 1.374

density

0.843 g/mL at 20 °C

suitability

in accordance for application

storage temp.

2-8°C

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

General description

2,4-Dinitrophenylhydrazine hydrochloric acid solution is a derivatization reagent.

Application

2,4-dinitrophenylhydrazine HCl solution was used in detecting Artemisinin derivatives usingf TLC silica gel sheets.[1] It may be used in determination of carbonyl groups.[2] It may also be used in a procedure for coating in situ silica gel with prepacked cartridges.[3]

Pictograms

FlameCorrosion
GHS02,GHS05

Signal Word

Danger

Hazard Statements

H225,H290,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

57.2 °F

Flash Point(C)

14.0 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000222197
0000213774
0000222197
0000213774
0000205996

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S B Tejada
International journal of environmental analytical chemistry, 26(2), 167-185 (1986-01-01)
A procedure for coating in situ silica gel in prepacked cartridges with 2,4-dinitrophenylhydrazine (DNPH) acidified with hydrochloric acid is described. The coated cartridge was compared with a validated DNPH impinger method for sampling organic carbonyl compounds (aldehydes and ketones) in
Jean-Robert Ioset et al.
PloS one, 4(9), e7270-e7270 (2009-10-01)
Malaria continues to be one of the major public health problems in Africa, Asia and Latin America. Artemisinin derivatives (ARTs; artesunate, artemether, and dihydroartemisinin) derived from the herb, Artemisia annua, are the most effective antimalarial drugs available providing rapid cures.
R Fields et al.
The Biochemical journal, 121(4), 587-589 (1971-02-01)
A method is described for determining carbonyl groups that is especially suitable for use with proteins and peptides. It involves the determination of the extinction at 370nm of a sample solution after adding 2,4-dinitrophenylhydrazine. The reaction of 2,4-dinitrophenylhydrazine with pyruvoylglycine
Camila Carrião M Garcia et al.
Journal of the American Chemical Society, 133(24), 9140-9143 (2011-05-25)
Acetaldehyde is an environmentally widespread genotoxic aldehyde present in tobacco smoke, vehicle exhaust and several food products. Endogenously, acetaldehyde is produced by the metabolic oxidation of ethanol by hepatic NAD-dependent alcohol dehydrogenase and during threonine catabolism. The formation of DNA
Shigehisa Uchiyama et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(17-18), 1282-1289 (2010-10-26)
Derivatization of carbonyl compounds with 2,4-dinitrophenylhydrazine (DNPH) is one of the most widely used analytical methods. In this article, we highlight recent advances using DNPH provided by our studies over past seven years. DNPH reacts with carbonyls to form corresponding
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