11966006001
Roche
TUNEL Dilution Buffer
pH 7.2-7.4 (20 °C), suitable for detection, pkg of 2 × 10 mL
Synonym(s):
TUNEL, transferase dUTP nick end labeling
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About This Item
UNSPSC Code:
41116100
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packaging
pkg of 2 × 10 mL
manufacturer/tradename
Roche
pH
7.2-7.4 (20 °C)
application(s)
detection
storage temp.
2-8°C
General description
The TUNEL dilution buffer can be used in combination with the in-situ cell death detection kits. It can be used to dilute terminal deoxynucleotidyl transferase (TdT) for the TUNEL reaction in special applications and also to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.
Application
TUNEL Dilution Buffer has been used:
- to dilute terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) enzyme (TdT) in preparing TUNEL reaction mixture for TUNEL critical commercial assays[1]
- to quantify apoptosis in rat kidney[2]
- to dilute the terminal deoxynucleotidyl transferase enzyme solution to identify DNA fragmentation in oligodendrocytes in vitro[3]
- for TUNEL assays[4]
TUNEL dilution buffer has been used in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays.[5]
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Storage conditions (working solution): Dilutions of TdT should be prepared immediately before use and should not be stored.
Keep TUNEL reaction mixture on ice until use.
The following concentrations should be taken as a guideline:
- ELISA: 100 mU/ml
- Western blot: 100mU/ml
Storage conditions (working solution): Dilutions of TdT should be prepared immediately before use and should not be stored.
Keep TUNEL reaction mixture on ice until use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Carc. 1B Inhalation
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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