MABT888

Anti-ZO-2 Antibody, clone Vli13

culture supernatant, clone Vli13, from rabbit

• Synonym(s): Tight junction protein ZO-2, Tight junction protein 2, Zona occludens protein 2, Zonula occludens protein 2, ZO-2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: Vli13, monoclonal
• species reactivity: mouse, rat, human
• technique(s): immunohistochemistry: suitable (paraffin)
• isotype: IgG
• NCBI accession no.: NP_035727.2
• UniProt accession no.: Q9Z0U1
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... TJP2(9414)

Description

General description

Tight junction protein ZO-2 (UniProt Q9Z0U1; also known as Tight junction protein 2, Zona occludens protein 2, Zonula occludens protein 2) is encoded by the Tjp2 (also known as Zo2) gene (Gene ID 21873) in murine species. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, -2, and -3 found at the submembranous domain of TJs in epithelia and endothelia. The 160 kDa ZO-2 contains three PDZ domains (PDZ1/a.a. 10-97, PDZ2/a.a. 287-365, PDZ3/a.a. 489-570) at its N-terminal end, followed by an SH3 domain (a.a. 584-649), a GUK/GK homology domain (a.a. 660-858), and a short PDZ-binding motif (TEL; a.a. 1165-1167) at the C-terminus. In addition to associating with various transmembrane junction proteins via its C-terminal PDZ-binding motif, ZO-2 is also involved in modulating gene expression and cell proliferation by interacting with a number of proteins in the nucleus. Evidence suggests that ZO-2 is also involved in stress response and cytoprotective mechanisms.

Specificity

Expected to react with human ZO-2 spliced isoforms A1, A2, C1, C2, 6, and 7, but not A3, reported by UniProt (Q9UDY2).

Immunogen

Epitope: C-terminal region.

Linear peptide corresponding to a C-terminal sequence of mouse ZO-2.

Application

Detect ZO-2 using this Anti-ZO-2 Antibody, clone Vli13 validated for use in Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ZO-2 in human heart and spleen tissue sections.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected ZO-2 in rat heart myocardium and brain tissue sections (Courtesy of Dr. Volkhard Lindner, Tufts University School of Medicine, ME, USA).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Immunohistochemistry in mouse heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ZO-2 in mouse heart tissue.

Target description

134.0 kDa (isoform A1), 117.8 kDa (isoform A2), 131.4 kDa (isoform C1), 115.2 kDa (isoform C2), 130.4 kDa (isoform 6), and 137.3 kDa (isoform 7) calculated.

Physical form

Rabbit monoclonal antibody in tissue culture supernatant with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2