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Key Documents

MABS444

Sigma-Aldrich

Anti-CLCA1 Antibody, clone 4D2.2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

purified antibody

Quality Level

clone

4D2.2
monoclonal

purified by

using protein G

species reactivity

mouse, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

General description

GST-tagged recombinant protein corresponding to an internal fragment from the C-terminal half of human CLCA1.

Specificity

Detects the 90 kDa processed/cleaved 4-transmembrane glycoprotein, but not the cleaved 37-41 kDa C-terminal fragment.

Application

Epitope: Internal (C-terminal half).

Quality

Evaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CLCA1 in 10 µg of human small intestine tissue lysate.

Physical properties

~83 kDa observed. 125 kDa glycosylated precursor form, 90 kDa and 37-41 kDa processed/cleaved fragements reported (Gruber, A.D., et al. (1998). Genomics. 54(2):200-214).

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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