MABS330

Anti-SFRP2 Antibody, clone 8H1

clone 8H1, from rat

• Synonym(s): Secreted frizzled-related protein 2, FRP-2, sFRP-2, Secreted apoptosis-related protein 1, SARP-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8H1, monoclonal
• species reactivity: mouse, horse
• species reactivity (predicted by homology): equine (based on 100% sequence homology), human (immunogen homology)
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_003004
• UniProt accession no.: Q96HF1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SFRP2(6423)

Description

General description

Secreted frizzled-related proteins (SFRPs) may play a critical role in carcinogenesis by inhibiting the Wnt/beta-catenin signaling pathway. Secreted frizzled-related protein 2 (SFRP2) may be useful to further investigate mammary tumorigenesis as its expression increases with tumor progression in canine mammary gland tumors. SFRP2 in mouse embryos has been found to cause severe shortening of the thoracic region. Studies have shown that this protein is the essential stem cell paracrine factor that is thought to regulate myocardial survival and repair damage in the aftermath of an ischemic injury.

Immunogen

Recombinant protein corresponding to human SFRP2.

Application

Detect SFRP using this rat monoclonal antibody, Anti-SFRP2 Antibody, clone 8H1 validated for use in western blotting & IHC.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A representative lot from an independent laboratory detected SFRP2 in RMG cell lysate and in ERU retinal tissue lysate (Hauck, S. M., et al. (2012). Journal of Proteomics. 75:4545-4554).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SFRP2 in equine retinal tissue (Hauck, S. M., et al. (2012). Journal of Proteomics. 75:4545-4554).

Quality

Evaluated by Western Blotting in mouse eye tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SFRP2 in 10 µg of mouse eye tissue lysate.

Target description

~ 33 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse eye tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable