MABN2394

Anti-Syntaxin-3B Antibody, clone 12E5

clone 12E5, from mouse

• Synonym(s): STX3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 12E5, monoclonal
• species reactivity: mouse, human, bovine
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001020478
• UniProt accession no.: Q64704
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: mouse ... Stx3(20908)

Description

General description

Syntaxin-3 (UniProt: Q64704; also known as STX3) is encoded by the Stx3 gene (Gene ID: 20908) in murine species. Syntaxin-3B is a single-pass type IV membrane protein that is specific for ribbon synapses of the retina. Stx3 gene codes for four different splice forms, Syntaxin 3A, 3B, 3C and 3D. Syntaxin 3A and 3B have an identical N-terminal domain but differ in the C-terminal half of the SNARE domain and the C-terminal transmembrane domain. These two domains are thought to be directly involved in synaptic vesicle fusion. Syntaxin 3 protein is found in all ribbon synapses of the retina. This type of synapse in the retina is formed by rod and cone photoreceptors, as well as by bipolar neurons. Syntaxin 3B has been shown to be important for synaptic vesicle exocytosis in retinal bipolar cells indicating a specific role for Syntaxin 3B in ribbon synapses. Syntaxin 3B can be phosphorylation at Thr14 by CAM Kinase II is shown to increase its binding to SNAP-25. (Ref.: Curtis, LB et al. (2008). J. Comp. Neurol. 510(5): 550-559; Liu, X et al. (2014). Mol. Cell. Neurosci. 60(1): 53-62).

Specificity

Clone 12E5 is a highly specific antibody against the B isoform of Syntaxin 3, which is abundantly expressed in photoreceptors of the retina.

Immunogen

GST-tagged recombinant murine Syntaxin 3, isoform B that lacks the transmembrane domain.

Application

Anti-Syntaxin-3B clone 12E5, Cat. No. MABN2394, is a highly specific mouse monoclonal antibody that targets Syntaxin 3B and has been tested in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Syntaxin-3B in human retina and mouse eye tissues.

Western Blotting Analysis: A representative lot detected Syntaxin-3B in Western Blotting applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

Immunoprecipitation Analysis: A representative lot detected Syntaxin-3B in Immunoprecipitation applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

Immunohistochemistry Analysis: A representative lot detected Syntaxin-3B in Immunohistochemistry applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

Immunocytochemistry Analysis: A representative lot detected Syntaxin-3B in Immunocytochemistry applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in human retinal tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Syntaxin-3B in 10 µg of human retinal tissue lysate.

Target description

~33 kDa observed; 32.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1