MABF3220

Anti- HPV16 Antibody, clone H16.V5

• Synonym(s): HPV-16, Major capsid protein L1
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: H16.V5, monoclonal
• mol wt: calculated mol wt 56.3 kDa; observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: virus
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; neutralization: suitable
• isotype: IgG2bκ
• Protein ID accession no.: N/A
• UniProt accession no.: P03101
• storage temp.: -10 to -25°C
• Gene Information: vaccinia virus ... L1(1489082)

Description

Specificity

Clone H16.V5 is a mouse monoclonal antibody that detects HPV16. It targets an epitope within the Major capsid protein L1. Might show some cross reactivity with HPV11.

Immunogen

Recombinant baculovirus-induced HPV-16 L1 Virus Like-Particles (VLPs)

Application

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b .

Tested Applications

ELISA Analysis: A representative lot of this antibody detected HPV16 protein in ELISA application (Data by Dr. J. Leitsch and with kind permission of ARTES Biotechnology GmbH, Germany. Recombinant HPV L1 antigens used were produced by ARTES Biotechnology GmbH).

Neutralizing: A representative lot detected HPV16 in Neutralizing application (Christensen, N.D., et al. (1996). Virology. 223(1):174-84; McClements, W.L., et al. (Virology). 289(2):262-8; Dias, D., et al. (2005). Clin Diagn Lab Immunol. 12(8):959-69; Smith, J.F., et al. (2008). Hum Vaccin. 4(2):134-42; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

ELISA Analysis: A representative lot detected HPV16 in ELISA application (Christensen, N.D., et al. (1996). Virology. 223(1):174-84; McClements, W.L., et al. (Virology). 289(2):262-8; Smith, J.F., et al. (2008). Hum Vaccin. 4(2):134-42; Panicker, G., et al. (2021). J Immunol Methods. 498:113136; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Major capsid protein L1 (UniProt: P03101) is encoded by the L1 gene (Gene ID: 1489082) in Human papillomavirus type 16. The Major Capsid Protein L1 of Human papillomavirus is the primary structural protein responsible for the formation of the viral capsid, that encapsulates the genomic DNA together with Minor Capsid Protein L2. The viral capsid is composed of 360 copies of the L1 protein forming 72 pentamers, that assemble into the icosahedral capsid structure, while the L2 protein localizes along the inner surface of the virion and plays a role in capsid stabilization through interaction with the Major Capsid Protein L1. The L1 protein facilitates the attachment of the virus to the host cell by interacting with heparan sulfate proteoglycans on the cell surface, a critical step for infection. Recombinantly expressed Major Capsid Protein L1 can spontaneously assemble into Virus Like-Particles (VLPs) and were used for the development of neutralizing antibodies that show high degree of Human papillomavirus type-specificity. Based on their oncogenic potential, Human papillomaviruses (HPVs) can be classified as low-risk HPV types, (e.g.: HPV6 and HPV11, the most common serotypes) which do not cause cancer but can lead to skin and genital warts, and high-risk HPV types (e.g.: HPV 16, 18, 31, 33, 35, 45, 52, 58), that are responsible for HPV related cancers. Vaccines targeting the L1 protein, have been developed and are effective in preventing infection with the most oncogenic HPV types by inducing a robust antibody response. (Ref.: Hareza, D. A., (2022) Int. J. Mol. Sci., 23, 1818.; Christensen, N.D., et al. (1990). J Virol. 64:11, 5678-81; Christensen, N.D., et al. (1996). Virology. 224(2):477-86; Brown, M.J., et al. (2014). Clin Vaccine Immunol. 21:4, 587-93)

Physical form

Purified mouse monoclonal antibody IgG2b in PBS without preservatives

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable