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MAB1940

Sigma-Aldrich

Anti-Fibronectin Antibody, cellular, clone DH1

clone DH1, Chemicon®, from mouse

Synonym(s):

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DH1, monoclonal

species reactivity

rat, guinea pig, human, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002026.2
NM_054034.2
NM_212474.1
NM_212475.1
NM_212476.1
NM_212478.1
NM_212482.1

UniProt accession no.

P02751

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
rabbit ... Fn1(100328589)
rat ... Fn1(25661)

Specificity

Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin.

Immunogen

Epitope: cellular
Purified fibronectin from Human A8387 fibrosarcoma cells.

Application

Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH.
Immunoblotting at 1:1,000

Indirect immunohistochemistry at 1:200-1:400

A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).

Final working dilutions must be determined by end user.

Physical form

Format: Purified
In PBS with 1% BSA and 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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