MAB19310

Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28

clone AF-28, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: AF-28, monoclonal
• species reactivity: bovine, rat, human, pig, mouse
• should not react with: guinea pig, horse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001135.2; NM_013227.2
• UniProt accession no.: P16112
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ACAN(176)

Description

General description

Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.

Specificity

Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.

Immunogen

Epitope: N-terminus FFGVG neoepitopes

FFGVGGEEDC-KLH peptide

Application

Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Inflammation & Autoimmune Mechanisms

Western blot

ELISA

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.

Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Cartilage, neural tube, and brain tissue

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable