GF177

APRIL, Human Recombinant

• Synonym(s): Tumor necrosis factor ligand superfamily member 13, TALL2, ZTNF2
• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.77

Properties

• impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Quality Level: 100
• UniProt accession no.: O75888
• shipped in: dry ice

Description

General description

APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor.
Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues.

Product Source: Protein is expressed in E.coli.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Quality

Measured by its ability to induce cell death in Jurkat cells.

Physical form

Product is presented in 10 mM Sodium Phosphate, pH 7.5 + 500 mM NaCl + 5% Trehalose and is filtered through a 0.2 micron filter before lyophilization.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1