GF142
Platelet Derived Growth Factor-AA Protein, Recombinant human
The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
Synonym(s):
PDGF-AA
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Recommended Products
biological source
human
Quality Level
Assay
>98% (SDS-PAGE and HPLC)
technique(s)
cell culture | mammalian: suitable
input
sample type neural stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... PDGFA(5154)
General description
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.
Product Source: E.coli
Application
For most in vitro applications, PDGF-AA exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): Fibroblasts, connective tissue.
Linkage
Replaces: GF017
Physical form
Lyophilized from a 10mM Acetic Acid solution.
Storage and Stability
The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -80°C. After a quick spin, reconstitute in 10mM Acetic Acid to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-AA should be stored in undiluted aliquots at -20°C for up to six months.
Analysis Note
Specific Activity: Human PDGF-AA is fully biologically active when compared to standards, as determined by colorimetric assay using BALB / 3T3 cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Certificates of Analysis (COA)
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