ABC20

Anti-Cystatin-C Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 5

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human, rat
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_000090
• UniProt accession no.: P01034
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Cystatin-C is a cysteine protease inhibitor in mammals, which is essential in protein degradation and keeping an equilibrium between inhibitors and proteases. It is thought to be a better marker for renal dysfunction and potential kidney injury than creatinine. Recently, Cystatin-C has been implicated in primary hypertension and may be a novel marker in patients. Cystatin-C has also been implicated in oxidative stress-induced apoptosis of CNS neurons and has been shown to inhibit amyloid-beta deposition in Alzheimer′s disease models.

~14 kDa observed.
An uncharacterized band may appear at ~62 kDa in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human Cystatin-C.

Application

Anti-Cystatin-C Antibody detects level of Cystatin-C & has been published & validated for use in WB, IH(P).

Immunohistochemistry (paraffin) Analysis: A 1:1000 dilution of a previous lot detected Cystatin-C in purkinje cells of normal rat cerebellum, neurons of normal rat frontal lobe, and neurons in normal rat midbrain.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Neurodegenerative Diseases

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Cystatin-C in 10 µg of human kidney tissue lysate.

Control
Human kidney tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable