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Millipore

His•Bind® Buffer Kit

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

storage temp.

2-8°C

General description

The His•Bind Buffer Kit is a set of pre-tested buffers designed for use with IDA-based His•Bind Resin for convenient, rapid one-step purification of proteins by metal chelation chromatography. Solutions are included for Ni2+ charging, binding, washing and elution of up to ten 2.5 ml columns.

Components

•2 × 80 ml8X Binding Buffer

•25 ml8X Wash Buffer

•50 ml4X Elute Buffer

•50 ml4X Strip Buffer

•20 ml8X Charge Buffer

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation

Target Organs

respiratory tract irritation

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

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Articles

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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