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BL21 Competent Cells - Novagen

Escherichia coli, rod shaped

Synonym(s):

BL21 cell line, Novagen competent cells

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About This Item

UNSPSC Code:
41106202
NACRES:
NA.81

product name

BL21 Competent Cells - Novagen, BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.

biological source

Escherichia coli

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

transformation efficiency: >2×107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlBL21 Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Other Notes

1. Phillips, T. A., Van Bogelen, R. A., and Neidhardt, F. C. (1984) J. Bacteriol.
159, 283–287.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pinku Sarmah et al.
Cell reports, 42(3), 112140-112140 (2023-02-27)
Signal-sequence-dependent protein targeting is essential for the spatiotemporal organization of eukaryotic and prokaryotic cells and is facilitated by dedicated protein targeting factors such as the signal recognition particle (SRP). However, targeting signals are not exclusively contained within proteins but can
John Maciejowski et al.
Developmental cell, 41(2), 143-156 (2017-04-26)
The spindle assembly checkpoint kinase Mps1 not only inhibits anaphase but also corrects erroneous attachments that could lead to missegregation and aneuploidy. However, Mps1's error correction-relevant substrates are unknown. Using a chemically tuned kinetochore-targeting assay, we show that Mps1 destabilizes microtubule attachments
Michio Nakaya et al.
Methods in molecular biology (Clifton, N.J.), 746, 317-327 (2011-05-25)
Heterotrimeric G proteins are composed of α, β, and γ subunits. G proteins can be activated by a large number of cell-surface hepathelical receptors and can transduce signals from these receptors to various intracellular signaling molecules. When G protein-coupled receptors

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