Native sphingomyelinase from Staphylococcus species. May be applied exogenously to initiate the sphingomyelin signaling pathway. Has very little activity on phosphatidylcholine.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of trinitrophenylaminolauryl (TNPAL)-sphingomyelin per min at 37°C, pH 7.4.
Physical form
In 250 mM phosphate buffer, 50% glycerol, pH 7.5.
Reconstitution
Following initial use, aliquot and refrigerate (4°C).
Other Notes
Galve-Roperh, I., et al. 1997. Brain Res. Mol. Brain Res.52, 90. Jarvis, W.D., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 73. Mathias, S., et al. 1993. Science259, 519. Raines, M.A., et al. 1993. J. Biol. Chem.268, 14572.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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