480450

NFF-2

A quenched fluorescence substrate for MMPs.

• Synonym(s): NFF-2, MCA-RPKPYANvaWMK(DNP)-NH₂
• Empirical Formula (Hill Notation): C₇₉H₁₀₅N₁₉O₁₉S
• Molecular Weight: 1656.86
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: yellow
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~325 nm; λ_em ~393 nm
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (k_cat/K_m = 5.9 x 10⁴ M^-1sec^-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; k_cat/K_m = 5 x 10⁴ M^-1s^-1), and MMP-9 (gelatinase-B; k_cat/K_m = 5.5 x 10⁴ M^-1s^-1).

A quenched fluorescent substrate for MMPs (excitation maximum: ~325 nm; emission maximum: ~393 nm). MMP-3 (stromelysin-1) hydrolyzes this substrate (k_cat/K_m = 59,400 M^-1s^-1) at a rate 60X faster than hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; k_cat/K_m = 54,000 M^-1s^-1), and MMP-9 (gelatinase-B; k_cat/K_m = 55,300 M^-1s^-1).

Biochem/physiol Actions

Cell permeable: no

Primary Target
Matrix Metalloproteinases (MMPs)

Product does not compete with ATP.

Reversible: no

kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)

Warning

Toxicity: Standard Handling (A)

Sequence

MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable