475954
MRN-ATM Pathway Inhibitor, Mirin
The MRN-ATM Pathway Inhibitor, Mirin, also referenced under CAS 299953-00-7, controls the biological activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex. This small molecule/inhibitor is primarily used for Cell Structure applications.
Synonym(s):
MRN-ATM Pathway Inhibitor, Mirin, (Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
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About This Item
Empirical Formula (Hill Notation):
C10H8N2O2S
CAS Number:
Molecular Weight:
220.25
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
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Quality Level
Assay
≥95% (HPLC)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
red-orange
solubility
DMSO: 10 mg/mL
ethanol: 3 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
A cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
A cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC50 = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Garner, K.M., et al. 2009. Nature Chem. Biol.5, 129.
Peterson, S., et al. 2009. Nature Chem. Biol.5, 130.
Dupre, A., et al. 2008. Nature Chem. Biol.4, 119.
Peterson, S., et al. 2009. Nature Chem. Biol.5, 130.
Dupre, A., et al. 2008. Nature Chem. Biol.4, 119.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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