407252

Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli

• Synonym(s): mIGF-I
• UNSPSC Code: 12352202

Properties

• Assay: ≥98% (SDS-PAGE and HPLC)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1 ng/μg Endotoxin (ng/μg IGF-I)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Full-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.

Biochem/physiol Actions

ED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.

Warning

Toxicity: Standard Handling (A)

Physical form

Sterile-filtered prior to lyophilization. Contains no additives.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.

Reconstitute with sterile H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffer before use.

Other Notes

Clemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res.14, 7873.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable