219374

Cetyltrimethylammonium Bromide, Molecular Biology Grade

Cationic detergent suitable for the isolation of high molecular weight DNA in plants and other organisms.

• Synonym(s): Cetyltrimethylammonium Bromide, Molecular Biology Grade, Cetrimide, CTAB
• Empirical Formula (Hill Notation): C₁₉H₄₂BrN
• CAS Number: 57-09-0
• Molecular Weight: 364.45
• MDL number: MFCD00011772
• UNSPSC Code: 12161902
• NACRES: NA.21

Properties

• grade: Molecular Biology
• Quality Level: 100
• Assay: ≥98% (TLC)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• aggregation number: 170
• color: white
• cation traces: heavy metals (as Pb): ≤0.001%
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 15-25°C
• InChI: 1S/C19H42N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(2,3)4;/h5-19H2,1-4H3;1H/q+1;/p-1
• InChI key: LZZYPRNAOMGNLH-UHFFFAOYSA-M

Description

General description

Cationic detergent suitable for the isolation of high molecular weight DNA in plants and other organisms.

Application

• Optimization of a high-throughput CTAB-based protocol for the extraction of qPCR-grade DNA from rumen fluid, plant and bacterial pure cultures.: This study details the optimization of a high-throughput protocol using Cetyltrimethylammonium Bromide (CTAB) for extracting high-quality DNA suitable for quantitative PCR (qPCR) from various biological samples. The optimized protocol is significant for molecular biology applications as it ensures efficient and reliable DNA extraction, which is crucial for downstream genetic analyses. (Minas et al., 2011).

Warning

Toxicity: Harmful (C)

Preparation Note

Heating to 30-35°C may be required for complete solubilization.

Reconstitution

Following reconstitution store at room temprature (20°C). Stock solutions are stable for up to 6 months at room temperature.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H315,H318,H335,H373,H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral - STOT SE 3
• Target Organs: Gastrointestinal tract, Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): 471.2 °F - closed cup
• Flash Point(C): 244 °C - closed cup