171259

AMPK Activator III, DHPO

The AMPK Activator III, DHPO modulates the biological activity of AMPK. This small molecule/inhibitor is primarily used for Activators/Inducers applications.

• Synonym(s): AMPK Activator III, DHPO, 1-oxo-2-(2H-pyrrolium-1-yl)-1H-inden-3-olate, AMPK Signaling Activator III
• Empirical Formula (Hill Notation): C₁₃H₉NO₂
• Molecular Weight: 211.22
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: >95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: orange
• solubility: DMSO: 10 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable AMPK activator (20 mg/kg/d i.p. for 21d) that has been shown to attenuate fasting blood glucose, improve glucose disposal, and correct dyslipidemia in genetic (leptin deficient, ob/ob) and dietary (high-fat-fed) mouse models of insulin resistance over controls. IP injections for 14 days increases skeletal muscle glucose uptake by > ~30% and ~70% compared to vehicle-treated lean and obese mice, respectively. Treatment with 100 µM of DHPO results in a small but significant AMPK (Thr¹⁷²) phosphorylation, and a concentration dependent increase in glucose uptake with a 2-fold increase over controls, in cultured myotubes. Furthermore, 20 nM Compound C demonstrates a 58% and 41% inhibition of DHPO-stimulated glucose up-take at concentrations of 50 µM and 100 µM DHPO, respectively.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in solution; reconstitute just prior to use.

Other Notes

Kandadi, M.R., et al. 2009. Biochem. Pharmacol.79, 623.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable