14-1054

Human recombinant histone H3.3-H4 tetramers

Human recombinant histone H3.3-H4 tetramers expressed in E Coli as fusion proteins with either an N-terminal FLAG or 6xHis epitopes & purified by Fast Protein Liquid Chromatography (FPLC).

• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: Escherichia coli
• recombinant: expressed in E. coli
• Assay: >98% (SDS-PAGE)
• manufacturer/tradename: Upstate^®
• concentration: 1 μg/μL
• technique(s): activity assay: suitable
• shipped in: dry ice

Description

General description

Human recombinant histone H3.3-H4 tetramers expressed in E Coli. as fusion proteins with either an N-terminal FLAG^® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).

Product Source: Human

The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The histone variant H3.3 replaces the conventional H3.1 histone in a wide range of nucleosomes in active genes, and are incorporated into chromatin independently of DNA synthesis. The structure of chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP) which requires high quality chromatin and essential tools such as histone proteins and appropriate enzymes to assemble chromatin in vitro.

Application

Research Category
Epigenetics & Nuclear Function

Suitable for in vitro chromatin assembly and enzyme activity assays

Physical form

In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 2 M NaCl, 0.5 mM PMSF and 1 mM DTT

Storage and Stability

Stable at -80ºC for 1 year from date of receipt.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable