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1.10649

Millipore

Bacillus subtilis (BGA) spore suspension

Indicator organism for the detection of antimicrobial inhibitors.

Synonym(s):

B. subtilis, Biological indicators

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15 X 2 ML
$149.00

$149.00

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15 X 2 ML
$149.00

About This Item

UNSPSC Code:
41116130
NACRES:
NA.84

$149.00

Please contact Customer Service for Availability
Request more information

Quality Level

100

form

liquid

technique(s)

microbiological culture: suitable

density

1 g/cm3

application(s)

detection
food and beverages
microbiology

storage temp.

2-8°C

suitability

Bacillus spp.

General description

Bacillus subtilis (BGA) spore suspension is used for the detection of antimicrobial inhibitors. The antimicrobial inhibitors test is conducted as an agar diffusion test. The spores of Bacillus subtilis (BGA) are the test organisms. The inhibitors inhibit the growth of the test bacteria. The inhibition of growth is indicated by a zone of inhibition measured between the edge of the piece of tissue and the limit of growth. Complete inhibition of growth with an inhibition zone of a minimum of 2 mm can be regarded as a positive result, and an inhibition zone of 1 to 2 mm as a dubious result, if the parallel controls have inhibition zones of approx. 6 mm.

Application

Bacillus subtilis (BGA) spore suspension is used for the detection of antimicrobial inhibitors in meat products by routine methods. BGA spore suspension is used along with the culture media test agar pH 6.0 (1.10663) and test agar pH 8.0 (1.10664) for the inhibitor test.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–2 of 2 Questions  

  1. In what medium are the spores suspended? How have these spores been stored?

    1 answer

    1. Unfortunately, the buffer formulation used to prepare the spores remains proprietary.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/691/09-102-1-pb-110649-engl.pdf

      Helpful?

  2. Can I know the concentration of the suspension solutions?

    1 answer

    1. Product 110649MM, Bacillus subtilis (BGA) spore suspension, contains viable spores of Bacillus subtilis DSM 618 at a concentration of 8E+06 - 5E+07 CFU/mL.

      Helpful?

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