1.09202

Cefixime Tellurite Supplement

for Escherichia coli, pkg of 10 vials, for the preparation of CT-SMAC Agar

• Synonym(s): E. coli Selective Supplement, Escherichia coli Selective Supplement, CT supplement
• UNSPSC Code: 41106212
• NACRES: NA.74

Properties

• description: GranuCult™ prime Sorbitol MacConkey (SMAC) Agar (1.00213)
• Quality Level: 200
• form: solid
• composition: Cefixime, 0.0025 mg per vial ; Potassium tellurite, 1.25 mg per vial
• packaging: pkg of 10 vials
• technique(s): microbiological culture: suitable
• application(s): food and beverages; microbiology
• storage temp.: 2-8°C
• suitability: Escherichia coli

Description

General description

CT-Supplement contains cefixime and potassium tellurite. It is used in combination with Sorbitol MacConkey Agar (SMCA) to enhance the isolation of Escherichia coli O157.

Application

CT-Supplement is an additive for the preparation of CT-SMAC Agar for the isolation and differentiation of enterohemorrhagic (EHEC) E. coli 0157:H7-strains from foodstuffs and stool.

Linkage

Replaces: 1092020017

Analysis Note

Appearance
- clearness:
clear
- colour: colourless
pH-value (25 °C): 5.3 - 5.7
Typical composition (per vial): Cefixim 0.025 mg; Potassium Tellurite 1.25 mg.
Growth promotion test in accordance with ISO 11133.
Supplement tested with base Art. 1.09207.
Inoculum on reference medium
- Escherichia coli ATCC 35150 (O157):
10 - 100
- Escherichia coli O157:H7 ATCC 43894: 10 - 100
- Escherichia coli O157:H7 ATCC 43895: 10 - 100
Inoculum
- Escherichia coli ATCC 11775 (WDCM 00090):
>10⁴
- Escherichia coli ATCC 25922 (WDCM 00013): >10⁴
- Staphylococcus aureus ATCC 6538 (WDCM 00032): >10⁴
- Staphylococcus aureus ATCC 25923 (WDCM 00034): >10⁴
Recovery on test medium
- Escherichia coli ATCC 35150 (O157):
≥ 50%
- Escherichia coli O157:H7 ATCC 43894: ≥ 50%
- Escherichia coli O157:H7 ATCC 43895: ≥ 50%
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
partly inhibition
- Escherichia coli ATCC 25922 (WDCM 00013): partly inhibition
- Staphylococcus aureus ATCC 6538 (WDCM 00032): total inhibition
- Staphylococcus aureus ATCC 25923 (WDCM 00034): total inhibition
Colony colour
- Escherichia coli ATCC 35150 (O157):
colourless
- Escherichia coli O157:H7 ATCC 43894: colourless
- Escherichia coli O157:H7 ATCC 43895: colourless
- Escherichia coli ATCC 11775 (WDCM 00090): pink
- Escherichia coli ATCC 25922 (WDCM 00013): pink
Incubation: 24 hrs.; 37 °C; aerobic.
Reference: Tryptic-soy-agar. Inhibited strains tested with three-loop-streak.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302 - H319 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P301 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3