1.04199

Methyl blue (C.I. 42780)

• Empirical Formula (Hill Notation): C₃₇H₂₇N₃Na₂O₉S₃
• CAS Number: 28983-56-4
• Molecular Weight: 799.80
• MDL number: MFCD00058509
• UNSPSC Code: 41116130
• NACRES: NA.21

Properties

• form: solid
• Quality Level: 200
• potency: >5000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥60% spectrophotometric assay
• pH: 5.0 (20 °C, 10 g/L in H₂O)
• solubility: 70 g/L
• bulk density: 670 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C37H29N3O9S3.2Na/c41-50(42,43)34-10-4-1-7-31(34)38-28-19-13-25(14-20-28)37(26-15-21-29(22-16-26)39-32-8-2-5-11-35(32)51(44,45)46)27-17-23-30(24-18-27)40-33-9-3-6-12-36(33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-3
• InChI key: TUHAIJABPUJAEY-UHFFFAOYSA-K

Description

General description

Methyl blue (C.I.42780), is a dry dye for the preparation of a color staining solution used for the polychrome staining of histological tissue sections of human origin. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Analysis Note

Dye content (spectrophotometrically): ≥ 60 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (Hydrochloric acid 0.005 mol/l): 595 - 605 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; hydrochloric acid 0.005 mol/l): 675 - 1125
TLC-Test: passes test
Loss on drying (110 °C): ≤ 7 %
Suitability for microscopy: passes test

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): 572.0 °F - Pensky-Martens closed cup
• Flash Point(C): > 300 °C - Pensky-Martens closed cup