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Key Documents

07-473-AF555

Sigma-Aldrich

Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor 555 Conjugate

from rabbit, ALEXA FLUOR 555

Synonym(s):

H3K4me3, H3.3B, Lys4 trimethylated, H3 histone, family 3A, Lys4 trimethylated, H3 histone, family 3B, Lys4 trimethylated, Histone H3, Lys4 trimethylated

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 555

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

trimethylation (Lys4)

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Specificity

Expected to react with most other vertebrate species based on highly conserved sequence homology.
This antibody only detects histone H3 when trimethylated on Lys4.

Immunogen

Epitope: Trimethylated Lys4 (H3K4me3).
Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys4 of histone H3.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected detected histone H3 Lys4 trimethylation in HeLa cells.
The unconjugated antibody (Cat. No. 07-473) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, and Western blotting applications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
This Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor 555 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3.

Quality

Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Lys4 trimethylation in HepG2 cells.

Target description

~16 kDa observed.

Physical form

Affinity purified.
Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2


Certificates of Analysis (COA)

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