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790326P

Avanti

Fluorescent Transfection Reagent

Avanti Research™ - A Croda Brand 790326P, powder

Synonym(s):

Fluorescent Transfection Reagent, Fluorescent Transfection Reagent, contains DOTAP:DOPE (1:1, w/w) + 0.1 wt.% NBD-DOPE

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.83

form

powder

packaging

pkg of 1 × 10 mg (790326P-10mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand 790326P

technique(s)

transfection: suitable

lipid type

transfection
cationic lipids

shipped in

dry ice

storage temp.

−20°C

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General description

Fluorescent transfection reagent has the cationic lipid 1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride (DOTAP) and the neutral lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine in the ratio 1:1 w/w along with 0.1 wt% N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) DOPE. NBD-DOPE is a fluorescence probe with NBD attached to headgroup of DOPE.

Biochem/physiol Actions

The DOTAP:DOPE with 0.1% NBD-DOPE liposome (LipNBD) reconstituted with transferrin called the transferrin liposome-nitrobenzoxadiazole (Tf-LipNBD) is useful as a probe. It provides improved image contrast in in vitro magnetic resonance imaging (MRI) of tumor cells. Fluorescent transfection reagent aids in understanding fibroplex membrane structure and fibroin interaction with cationic lipids. N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) is used in model membranes studies and is useful in visualizing membrane domains.

Packaging

5 mL Clear Glass Sealed Ampule (790326P-10mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Storage Class Code

11 - Combustible Solids

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The influence of NBD fluorescent probe on model membranes containing POPC and DPPC
Weng CJ, et al.
Molecular Membrane Biology, 33(1-2), 23-28 (2016)
Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts
Shan L, et al.
Molecular Imaging, 6(2), 7290-2007 (2007)
Efficiency data of intracellular recombinant protein delivery using cationic lipid coated silk fibroin particle
Kim WJ, et al.
Data in Brief, 13, 683-691 (2017)
Woo-Jin Kim et al.
Biomaterials, 122, 154-162 (2017-01-26)
Directly delivering therapeutic proteins into cells has promise as an intervention without side effects for protein deficiencies caused by genetic defects. However, as negatively charged macromolecules, proteins require carriers for achieving cellular uptake and maintaining their activity in the cytoplasm.

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