L032000

DCI Activator

0.25 M

• Synonym(s): DCI activator solution
• UNSPSC Code: 41116105
• NACRES: NA.21

Properties

• type: for DNA synthesis; for DNA synthesis
• product line: Proligo Reagents
• form: liquid
• storage condition: dry at room temperature
• concentration: 29-32 g/L (DCI content, titration)
• impurities: ≤30 ppm water content (Karl Fischer); ≤30 ppm water content (Karl Fischer)
• color: clear; clear

Description

General description

Activator solution, containing powerful activators like 4,5-dicyanoimidazole(DCI), 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) or5-(Ethylthio)-1H-tetrazole (ETT) in acetonitrile, are mixed with solutions ofphosphoramidites during the extension step. The activator reacts with theamidite group to form a highly reactive intermediate. The intermediate thenforms an internucleotide bond with the detritylated 5′hydroxyl group of thegrowing oligonucleotide chain.

Preparation Note

Dicyanoimidazole / Acetonitrile 3/100 (w/v)

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H302 + H312 + H332,H315,H318,H335
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 41.0 °F
• Flash Point(C): 5 °C