806005

APN-Amine HCl

95%

• Synonym(s): 3-(4-(4-(Aminomethyl)-1H-1,2,3-triazol-1-yl)phenyl)propiolonitrile hydrochloride
• Empirical Formula (Hill Notation): C₁₂H₁₀N₅Cl
• Molecular Weight: 259.69
• UNSPSC Code: 12352200
• PubChem Substance ID: 329768982

Properties

• Quality Level: 100
• Assay: 95%
• form: powder
• reaction suitability: reagent type: cross-linking reagent
• storage temp.: 2-8°C
• SMILES string: N#CC#CC1=CC=C(N2C=C(CN)N=N2)C=C1.[H]Cl
• InChI: 1S/C12H9N5.ClH/c13-7-1-2-10-3-5-12(6-4-10)17-9-11(8-14)15-16-17;/h3-6,9H,8,14H2;1H
• InChI key: JZGAUVVBWJHLGN-UHFFFAOYSA-N

Description

Application

APN-Amine is a bifunctional crosslinker for thiol-to-carboxyl coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. The amino group readily reacts with activated carboxylic acids (e.g. NHS-esters) in basic conditions to produce stable amide bonds. The APN group can be used for coupling with free thiols (e.g. labeling of free cysteines in proteins).

Other Notes

Standard protein labeling procedure (cysteine labeling):

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

*Note: avoid thiol-containing buffers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable