T0195

α-Bungarotoxin-tetramethylrhodamine

from Bungarus multicinctus (Formosan Banded Krait), Nicotinic acetylcholine receptor antagonist

• Synonym(s): TMR-α-BTX
• UNSPSC Code: 12352116
• NACRES: NA.77
• MDL number: MFCD00286495

Properties

• Product Name: α-Bungarotoxin-tetramethylrhodamine from Bungarus multicinctus (Formosan Banded Krait),
• mol wt: ~8,500
• Quality Segment: 100
• color: red
• solubility: H₂O: soluble
• storage temp.: −20°C

Description

Application

α-Bungarotoxin-tetramethylrhodamine from Bungarus multicinctus (Formosan Banded Krait) has been used in immunofluorescence staining (1:200) for identification of neuromuscular junction in experimental models. It has also been used to block muscle contraction in myotome cells.

α-Bungarotoxin-tetramethylrhodamine from Bungarus multicinctus was used at a dilution of 1:220 to identify the distribution of nicotinic acetylcholine receptors at neuromuscular junctions of longitudinal sections of rat gastrocnemius muscle.

Biochem/physiol Actions

Useful for detecting the distribution of nicotinic acetylcholine receptors at neuromuscular junctions.

α-Bungarotoxin is a high-affinity antagonist for nicotinic acetylcholine receptors (AChRs) from muscle but not the neurons. Labeling α-Bungarotoxin with tetramethylrhodamine enables the detection of bungarotoxin-binding receptors at neuromuscular junctions.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)