SRP5158

14-3-3 θ, untagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): 14-3-3, 14-3-3 θ, 1C5, HS1, YWHAQ
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~29 kDa
• NCBI accession no.: NM_006826
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... YWHAQ(10971)

Description

General description

14-3-3θ (also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3θ plays an important role in controlling apotopsis. Induced expression of 14-3-3θ protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3θ has been observed to mediate nucleocytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable