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SRP0700

Sigma-Aldrich

STAT5B Protein

recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in baculovirus infected Sf9 cells

Assay

70-90% (SDS-PAGE)

form

frozen liquid

mol wt

~115 kDa

concentration

0.2 μg/μL

accession no.

BC065227

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... STAT5B(6777)

Application

Useful for Western blot.

Biochem/physiol Actions

Recombinant full-length human STAT5B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STAT5B or signal transducer and activator of transcription 5B is a member of the STAT family of transcription factors. STATs are phosphorylated by the receptor associated kinases and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B is activated in response to a variety of cytokines (1). The JAK/STAT pathway mediates a key signal from the somatic gonad that regulates male germline sexual development (2). STAT5B is critical for maintenance of tolerance in vivo and it is proposed that STAT5B is probably activated by IL2R.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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