This medium has not been evaluated for freezer storage. The thawing process may cause precipitation of some salts. The end user may determine the suitability experimentally by freezing a small test volume. It is advisable to thaw the product slowly to minimize the chance of precipitation. Thaw the sample slowly, overnight in a refrigerator., if possible It will very likely be suitable if the resulting thawed medium is clear, colorless, and free of particulate matter. Do not add serum or supplements prior to freezing the test sample.
S0146
Schneider’s Insect Medium
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture
Synonym(s):
Schneider’s Drosophila Medium
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About This Item
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Product Name
Schneider′s Insect Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | insect: suitable
suitability
suitable for culture of other dipteran cell lines (Originally developed for the culture of Drosophila cells)
components
NaHCO3: 0.4 g/L
glucose: 2 g/L (Dextro)
L-glutamine: 1.8 g/L
shipped in
ambient
storage temp.
2-8°C
Application
Originally developed for the culture of Drosophila; suitable for culture of other dipteran cell lines.
Schneider′s insect medium has been used:
- to culture Drosophila neuronal cell line (BG3-c2 cells)[1]
- to cultivate subcultures of Leishmania major for promastigote growth inhibition assay[2]
- to seed purified ookinetes with Drosophila melanogaster S2 cells to assess the effect of the avermectin compound on cell development and to promote oocyte development[3]
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This Item | |||
|---|---|---|---|
| form liquid | form powder | form liquid | form liquid |
| suitability suitable for culture of other dipteran cell lines (Originally developed for the culture of Drosophila cells) | suitability - | suitability - | suitability - |
| technique(s) cell culture | insect: suitable | technique(s) cell culture | insect: suitable | technique(s) cell culture | insect: suitable | technique(s) cell culture | insect: suitable |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility sterile-filtered |
| components NaHCO3: 0.4 g/L | components glucose: 2 g/L (Dextro) | components glucose: 10 g/L (Dextro) | components NaHCO3: 0.35 g/L |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is it possible to freeze this medium ? I'll need to use only a few millilitres for each dissections of fly brains so it'll be great if I could freeze it in smaller containers to extend the shelf life period.
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