The Polyinosinic-Polycytidylic acid Sodium Salt (P1530) is a double-stranded homopolymer with variable molecular weights. The variable range has been observed at 400-1250 base pairs; however, this may vary from lot to lot.
P1530
Polyinosinic–polycytidylic acid sodium salt
TLR ligand tested
Synonym(s):
Poly (I:C), Poly(I) • Poly(C)
Sign Into View Organizational & Contract Pricing
All Photos(4)
About This Item
Pricing and availability is not currently available.
Recommended Products
Quality Level
Assay
≥99% (less than 1% free nucleotides, TLC)
form
lyophilized powder
storage condition
desiccated
solubility
H2O: 10 mg/mL
storage temp.
−20°C
SMILES string
[Na].[P](=O)(OC[C@H]3O[C@H]([C@@H]([C@@H]3O)O)[n]4c5ncnc(c5nc4)O)(O)O.[P](=O)(OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)N2C=CC(=NC2=O)N)(O)O
InChI
1S/C10H13N4O8P.C9H14N3O8P.Na/c15-6-4(1-21-23(18,19)20)22-10(7(6)16)14-3-13-5-8(14)11-2-12-9(5)17;10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(20-8)3-19-21(16,17)18;/h2-4,6-7,10,15-16H,1H2,(H,11,12,17)(H2,18,19,20);1-2,4,6-8,13-14H,3H2,(H2,10,11,15)(H2,16,17,18);/t
InChI key
GETQKWBYQAUFQM-VRQAYDGLSA-N
Looking for similar products? Visit Product Comparison Guide
Application
TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.
Biochem/physiol Actions
Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.
Other Notes
Double-stranded homopolymer.
Preparation Note
The product requires ionic strength to maintain the double-strand structure. To prevent denaturation, reconstitute in solutions with physiological salt concentrations (e.g., saline solution). Reconstitution may require heating (50 °C) and cooling to achieve re-annealing.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Yipeng Ren et al.
Fish & shellfish immunology, 70, 720-730 (2017-09-30)
Toll-like receptors, the best known pattern recognition receptors, play important roles in recognizing non-self molecules and binding pathogen-associated molecular patterns in the innate immune system. In the present research, the cDNA and protein characterization of the TLR signalling pathway genes
Pei-Hui Wang et al.
Nucleic acids research, 46(8), 4054-4071 (2018-03-17)
STING is a core adaptor in innate nucleic acid sensing in mammalian cells, on which different sensing pathways converge to induce type I interferon (IFN) production. Particularly, STING is activated by 2'3'-cGAMP, a cyclic dinucleotide containing mixed phosphodiester linkages and
Anutosh Paria et al.
Developmental and comparative immunology, 78, 71-82 (2017-09-19)
MDA5 is the pivotal member of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) and is reported to play a crucial role in type I IFN-mediated responses against pathogen-associated molecular patterns (PAMPs), especially nucleic acids. In this study, we have
Baohong Wu et al.
Signal transduction and targeted therapy, 4, 11-11 (2019-05-03)
UTX (also known as KDM6A), a histone 3 lysine 27 demethylase, is among the most frequently mutated epigenetic regulators in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Recent studies have suggested that UTX mutations promote MDS and AML by
Fei Yuan et al.
Antiviral research, 147, 1-10 (2017-05-04)
The present study is focused on investigating the immunoprotective effects of CpG-ODN/Poly(I:C) combined with the viral glycoprotein gp90 protein against reticuloendotheliosis virus (REV) infection in chickens. REV's gp90 gene was amplified from the REV-infected cells and expressed in Escherichia coli
-
What is the molecular weight of Polyinosinic-Polycytidylic acid Sodium Salt (P1530)?
1 answer-
Helpful?
-
-
What is the length of the poly(I).(C)?
1 answer-
The product is a double-stranded homopolymer without a specified length. Historical data have shown a broad lot-to-lot variability with an approximate range of 200 - >1000 bp length.
Helpful?
-
-
What is the length of the Poly/I).poly(C)?
1 answer-
The product is double-stranded homopolymer without specified length,. Historical data have shown broad lot to lot variability with an approximately range of 200 - >1000 bp in length.
Helpful?
-
-
Could you please elucidate about the purity of the compound and if its sutible for human use or GMP grade?
1 answer-
The minimum purity specification for this item is 99%. However, this product it is not suitable for human use or GMP applications. Unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Please see the link to below to review addtional information regarding our various quality levels:
https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/m-clarity-programHelpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
