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P1259

Sigma-Aldrich

Phytase from wheat

≥0.01 unit/mg solid

Synonym(s):

6-Phytase, Phytate 6-phosphatase, myo-Inositol-hexakisphosphate 6-phosphohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

form

powder

Quality Level

specific activity

≥0.01 unit/mg solid

storage temp.

−20°C

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General description

Phytase catalyzes the hydrolysis of phytic acid to inositol and free orthophosphate. [1]

Application

Phytase has been used in a study to quantify the number of feedstuffs showing significant phytase activity. [2] It has also been used in a study to investigate the effect of adjusting the pH to 5.0 (optimum for wheat phytase) during whole wheat bread making on phytase activity. [3]

Biochem/physiol Actions

Wheat phytase can degrade inositol phosphate-6 and 5 at a pH of 4 and 5. [4]

Quality

Crude

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphorus from 1.5 mM phytate per min at pH 5.15 at 55 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Chandra Shekar Mootapally et al.
Journal of molecular microbiology and biotechnology, 26(4), 252-260 (2016-05-14)
Phytases have been widely used as animal feed supplements to increase the availability of digestible phosphorus, especially in monogastric animals fed cereal grains. The present study describes the identification of a full-length phytase gene of Prevotella species present in Mehsani
Elsa Arcalis et al.
Plant molecular biology, 83(1-2), 105-117 (2013-04-05)
Many plant-based systems have been developed as bioreactors to produce recombinant proteins. The choice of system for large-scale production depends on its intrinsic expression efficiency and its propensity for scale-up, post-harvest storage and downstream processing. Factors that must be considered
J C González-Vega et al.
Journal of animal science, 91(10), 4807-4816 (2013-08-15)
An experiment was conducted to test the hypothesis that values for apparent total tract digestibility (ATTD) of Ca in pigs are influenced by endogenous Ca lost from the gastrointestinal tract. The objective was to determine the endogenous loss of Ca
Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs
Eeckhout, W. and M. Paepe
Anim. Feed Sci. Technol., 47, 19-29 (1994)
Katrine Pontoppidan et al.
Archives of animal nutrition, 66(6), 431-444 (2012-10-27)
Phytases (EC 3.1.3) are widely used in animal feed to increase the availability of phosphorus and decrease the anti nutritive effect of myo-inositol hexakisphosphate (InsP₆). The aim of this work was to investigate the stereospecific degradation of InsP₆ in vitro

Questions

1–2 of 2 Questions  
  1. What would be a molecular weight? And how much purity is the powder?

    1 answer
    1. Unfortunately, this product is not tested for purity and the molecular weight has not been determined. This enzyme is sourced from Wheat. There is no molecular weight listed for Phytase from Triticum. Please see the link below to review the PRENDA profile for this enzyme.
      https://www.brenda-enzymes.org/all_enzymes.php?ecno=3.1.3.26&table=Molecular_Weight#TAB

      Helpful?

  2. What is the microbe that was used to produce the enzyme?

    1 answer
    1. The method of preparation of this product consists of a water extraction of wheat bran, concentration, and lyophilization. It is a very crude preparation. It is not from a recombinant source.

      Helpful?

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