P0072

Anti-phospho-PDCD4 (pSer⁶⁷) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Neoplastic transformation inhibitor, Anti-Nuclear antigen H731, Anti-Programmed cell death 4, Anti-Protein 197/15a
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~51 kDa
• species reactivity: rat (predicted), mouse (predicted), human
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells starved for 48 hours and then treated with 20% FCS; western blot: 1-2 μg/mL using lystes of HEK-293T cells starved for 48 hours and then treated with 20% FCS
• UniProt accession no.: Q53EL6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer67)
• Gene Information: human ... PDCD4(27250) , SELP(6403) ; mouse ... Pdcd4(18569)

Description

General description

Programmed cell death 4 (PDCD4) also known as nuclear antigen H731-like, Protein 197/15a, neoplastic transformation inhibitor protein), is a tumor suppressor protein that is lost in progressed carcinomas of lung, breast, colon and prostate.

Application

Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Programmed cell death 4 (PDCD4) suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex and by competing with eIF4G, a second component of the translation initiation complex, for binding to eIF4A. In response to mitogens, PDCD4 was rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF-β (TRCP). It has been shown that the protein is specifically phosphorylated on Ser67 and Ser457 by Akt, both in vitro and in vivo. This phosphorylation causes nuclear translocation of PDCD4.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves