MTOX1097

MRP7 Knockout Caco-2 Cells

human male colorectal tissue (Source Disease: colon adenocarcinoma)

• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: human male colorectal tissue (Source Disease: colon adenocarcinoma )

Properties

• Product Name: MRP7 Knockout Caco-2 Cells, one vial
• biological source: human male colorectal tissue (Source Disease: colon adenocarcinoma )
• Quality Segment: 200
• form: liquid
• technique(s): drug transporter assay: suitable, permeability assay: suitable
• application(s): ADME/TOX
• storage temp.: −196°C
• Gene Information: human ... ABCC10(89845)

Description

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP6 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC10 (MRP6) efflux transporter.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional knockout of the MRP6 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP6 substrate
• The vial format enables the MRP6 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Legal Information

ADME/Tox Cell Lines License

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable