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M8570

Sigma-Aldrich

Methylated Control DNA

50 ng/μL

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About This Item

UNSPSC Code:
23201100
NACRES:
NA.55
Pricing and availability is not currently available.

form

liquid

Quality Level

200

concentration

50 ng/μL

color

colorless

storage temp.

−20°C

Related Categories

General description

Fully methylated Jurkat DNA. 5-methyl-cytidine (5mC) content determined by mass spectrometry. See lot specific certificate of analysis or vial label for 5mC content of each lot.

Application

Methylated Control DNA has been used as a negative control in bisulfite sequencing of embryonic genomic DNA.[1]

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000254601
0000254601
SLCK5485
SLCL8936
SLCK7031

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Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts
Zhang D, et al.
Nucleic Acids Research, 45(6), 3102-3115 (2016)

Questions

1–3 of 3 Questions  

  1. What buffer is the Methylated Control DNA dissolved in?

    1 answer

    1. This product is dissolved in a TE buffer consisting of 10 mM Tris-HCl with 1 mM EDTA.

      Helpful?

  2. It has been written 50 ng/ul for concentration. What is the total volume (as ul or ml) and total probe amount (as ug or mg) in the vial. Could you inform me, please

    1 answer

    1. The total volume is 16ul.

      Helpful?

  3. What is the percentage of 5-Methylcytosine?

    1 answer

    1. As mentioned on the product detail page, the % 5mC content is reported in the lot specific Certificate of Analysis, as well as the product label. The specification for 5mC is >/= 4.5%. The historical average is approximately 6.3%. Please see the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/M8570-1VLSLCK7031.pdf

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