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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Minimum Essential Medium Eagle, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: yes
Hanks’ salts (2% CO₂): yes
L-glutamine: yes
Earle’s salts (5% CO₂): no
NaHCO₃: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

Application

Minimum Essential Medium Eagle (MEM) has been used:

as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures[1]
to culture Müller cells from guinea pigs

Preparation Note

Formulated to contain 10.6 grams of powder per liter of medium.

Supplement with 0.35 g/L sodium bicarbonate.

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This Item	M1018	M0644	M8042
Sigma-Aldrich M4642 Minimum Essential Medium Eagle	Sigma-Aldrich M1018 Minimum Essential Medium Eagle	Sigma-Aldrich M0644 Minimum Essential Medium Eagle	Sigma-Aldrich M8042 Minimum Essential Medium Eagle
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form powder	form liquid
components glucose: yes Hanks’ salts (2% CO₂): yes L-glutamine: yes Earle’s salts (5% CO₂): no NaHCO₃: no phenol red: yes sodium pyruvate: no stable glutamine: no HEPES: no	components phenol red: yes NaHCO₃: no HEPES: no sodium pyruvate: no Hanks’ salts (2% CO₂): yes L-glutamine: yes	components NaHCO₃: no L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: yes	components Hanks’ salts (2% CO₂): no glucose: yes sodium pyruvate: yes phenol red: yes HEPES: no L-glutamine: no stable glutamine: no Earle’s salts (5% CO₂): yes NaHCO₃: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
MEM, classic media, classical media, FBS	MEM, classic media, classical media, FBS	classic media, classical media, FBS, hybrid cells	classic media, classical media, MEM-alpha, classic media, classical media, FBS

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Storage Class

11 - Combustible Solids

wgk

WGK 1

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