Key Documents

Methylene Blue hydrate

Name: Methylene Blue hydrate
Brand: SIGMA

suitable for nucleic acid staining, BioReagent

Synonym(s):

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₈ClN₃S · xH₂O

CAS Number:

122965-43-9

Molecular Weight:

319.85 (anhydrous basis)

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

57654386

EC Number:

200-515-2

Beilstein/REAXYS Number:

3599847

MDL number:

MFCD00150006

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

grade

Molecular Biology

Quality Segment

100

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

water: 1 mg/mL (dark blue)

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

Description

General description

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Features and Benefits

Less hazardous than staining with ethidium bromide
Viewable in visible light
Fast and easy to use

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	66720	PHR3838	1428008
Sigma-Aldrich M4159 Methylene Blue hydrate	Sigma-Aldrich 66720 Methylene Blue hydrate	Supelco PHR3838 Methylene Blue	USP 1428008 Methylene blue
description suitable for nucleic acid staining, BioReagent	description purum p.a., suitable for microscopy, ≥97.0% (calc. based on dry substance, AT)	description certified reference material, pharmaceutical secondary standard	description United States Pharmacopeia (USP) Reference Standard
grade Molecular Biology	grade purum p.a.	grade pharmaceutical secondary standard, certified reference material	grade pharmaceutical primary standard
suitability suitable for nucleic acid staining	suitability suitable for microscopy	suitability -	suitability -
technique(s) nucleic acid detection: suitable	technique(s) -	technique(s) -	technique(s) -
form powder	form powder	form -	form -
solubility water: 1 mg/mL (dark blue)	solubility ethanol: 0.01 g/10 mL, blue to very deep blue	solubility -	solubility -
Quality Level 100	Quality Level 200	Quality Level 300	Quality Level -

Still not finding the right product?

Explore all of our products under Methylene Blue hydrate

Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.