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Medium 199

Name: Medium 199
Brand: SIGMA

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

M199 Medium, TCM 199

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.0-7.6 (25 °C, 209 g/L)

components

sodium pyruvate: no
L-glutamine: no
NaHCO₃: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO₂): yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

to culture rumen epithelial cells from yearling Speckle Park beef heifers
as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries
as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)

Preparation Note

Supplement with 0.1 g/L L-glutamine.

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This Item	M3769	M2520	M0393
Sigma-Aldrich M2154 Medium 199	Sigma-Aldrich M3769 Medium 199	Sigma-Aldrich M2520 Medium 199	Sigma-Aldrich M0393 Medium 199
description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture	description HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture	description With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form liquid	form powder	form powder	form powder
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility -	sterility -	sterility -
components sodium pyruvate: no L-glutamine: no NaHCO₃: yes HEPES: no phenol red: yes Earle’s salts (5% CO₂): yes	components L-glutamine: no phenol red: no HEPES: no sodium pyruvate: no Earle’s salts (5% CO₂): yes NaHCO₃: no	components L-glutamine: yes phenol red: yes Earle’s salts (5% CO₂): yes NaHCO₃: no sodium pyruvate: no HEPES: 25 mM	components HEPES: no Hanks’ salts (2% CO₂): yes sodium pyruvate: no NaHCO₃: no L-glutamine: yes phenol red: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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