This product should be reconstituted at 1 mg/ml in 100 sodium phosphate buffer, pH 7.2. Please see the Product Datasheet below, located in the 'DOCUMENTATION' section under 'More Documents':
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/149/457/l9656enz.pdf
L9656
Lipoprotein Lipase from Burkholderia sp.
lyophilized powder, ≥50,000 units/mg solid
Synonym(s):
Diacylglycerol acylhydrolase, Diacylglycerol lipase, Lipoprotein Lipase from Pseudomonas sp.
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About This Item
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biological source
Burkholderia spp.
Quality Level
form
lyophilized powder
specific activity
≥50,000 units/mg solid
storage temp.
−20°C
General description
Lipoprotein Lipase (LPL) is a glycerol ester hydrolase. Several bacteria that produce LPL, belongs to the genus Pseudomonas, Serratia and Mucor.[1]
Lipoprotein lipase hydrolyzes triglycerides in plasma lipoproteins causing release of fatty acids for metabolic purposes in muscles and adipose tissue. [2]
Application
Lipoprotein Lipase from Burkholderia sp. has been used in the subcellular fractionation of mussel Mytilus galloprovincialis to identify the biomolecule attached to cytosolic fraction of okodaic acid.[3] It has also been used to test its effect on serum amyloid A induced granulocyte colony-stimulated factor (G-CSF) expression in response to bacterial lipoprotein.[4]LipoproteinLipase from Burkholderia sp. is suitable for the synthesis ofdiarylmethanols, a precursor for the synthesis of pharmaceutical compounds.
Biochem/physiol Actions
Due to its lipolytic activity, lipoprotein lipase was shown to effectively block the spread of hepatitis C virus into healthy cells. [7]
Lipoprotein Lipase (LPL) hydrolysis triacylglycerol moieties in chylomicron and low density lipoproteins.[1][8] LPL attaches lipoprotein to the vessel wall and facilitates their uptake.[8] Abnormalities in LPL is associated with Alzheimer′s disease, atherosclerosis, obesity and chylomicronaemia.[9]
Lipoprotein lipase belongs to the family of triglyceride lipases. It hydrolyses triglycerides in triglyceride-rich ApoB-containing lipoproteins.
Unit Definition
One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 °C using p-nitrophenyl butyrate as substrate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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