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L9655

Linoleic Acid-Oleic Acid-Albumin, 100x

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Fatty Acid-Albumin-complexes

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352211
Form:
liquid
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biological source

protein from bovine

Quality Segment

sterility

sterile-filtered

product line

BioReagent

form

liquid

composition

linoleic acid and oleic acid, 2 mol/mol albumin (each)

concentration

100 mg/mL BSA in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

General description

Fatty Acid-Albumin-complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) is beneficial to the growth of a variety of cell types (e.g., baby hamster kidney (BHK) hybridoma). A mixture of poly-unsaturated and monosaturated fatty acids (i.e., linoleic acid and oleic acid) produces synergistic effect when used as a media supplement.

Application

Linoleic Acid-Oleic Acid-Albumin, 100x has been used:
  • to induce acute hepatocellular stress in primary hepatocytes [1]
  • to induce oxidative stress in cytotrophoblasts[2]
  • to induce lipid droplet accumulation in HepG2 cells[3]


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This Item
L9530L8384O1383
Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

lyophilized powder

form

viscous liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

biological source

protein from bovine

biological source

-

biological source

bovine plasma

biological source

palm oil

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

γ-irradiated

sterility

-


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Questions

1–2 of 2 Questions  
  1. what kind of DPBS is used for the solution? +/- Magnesium and or Calcium?

    1 answer
    1. This product is made with modified DPBS without Mg or Ca.

      Helpful?

  2. What is the molarity of the solution for oleic acid and linoleic acid?

    1 answer
    1. The concentration of both Oleic and Linoleic acid in this product is 9.4 mg/L. This converts to 3.327 uM and 3.351 uM, respectively. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/239/110/l9655pis.pdf

      Helpful?

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