The excitation and emission wavelengths for this product are approximately 553 nm and 635 nm, respectively. The 488 nm blue laser is not suitable for excitation.
L3030
Latex beads, carboxylate-modified polystyrene, fluorescent red
aqueous suspension, 2.0 μm mean particle size
Synonym(s):
Fluorescent latex beads
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About This Item
Pricing and availability is not currently available.
form
aqueous suspension
contains
0.1% NaN3
composition
Solids, 2.5%
technique(s)
cell based assay: suitable
mean particle size
2.0 μm
fluorescence
λex ~553 nm; λem ~635 nm
application(s)
cell analysis
Application
Carboxylate-modified polystyrene latex beads have been used to collect morphofunctional data about the immune systems of Carabus lefebvrei, a helicophagous Italian endemic ground beetle often used as an indicator of the habitat quality of the Apennines mountain forests. Carboxylate-modified polystyrene latex beads have also been used to inform comparative studies on immunity defense mechanisms of adults and larvae of the coleopteran Cetonischema aeruginosa and to investigate the phagocytic activity of cultured rat thyroid cells.
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| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - |
| fluorescence λex ~553 nm; λem ~635 nm | fluorescence λex ~575 nm; λem ~610 nm | fluorescence λex ~575 nm; λem ~610 nm | fluorescence λex ~520 nm; λem ~540 nm |
| form aqueous suspension | form aqueous suspension | form aqueous suspension | form aqueous suspension |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) - |
| composition Solids, 2.5% | composition Solids, 2.5% | composition Solids, 2.5% | composition Solids, 2.5% |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the spectrum of these red beads? In particular, is the 488nm blue laser suitable for their excitation?
1 answer-
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What is the correct storage temperature?
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This product may be stored at room temperature.
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Is the 2.4% wet weight concentration in mg o g
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The 2.5% composition referenced on this product page is the weight/weight concentration of beads in water. Typically, the percentage is reported in grams - in this case, 2.5 grams of beads per 100 grams of water. However, this also applies to milligrams - in this case, 2500 milligrams per 100,000 milligrams of water. The concentration of this product will range from 2.4 - 2.6%. This information is reported in the lot specific Certificate of Analysis. Please see the link below to review a sample or lot speciific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/l3030#product-documentationHelpful?
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What is the concentration of Beads in 1 ml?
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The weight/weight concentration is 2.5%, with a particle count of approximately 4.57x10^10/ml.
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