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H7379

Sigma-Aldrich

Hemoglobin human

lyophilized powder

Synonym(s):

Hb

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About This Item

CAS Number:
9008-02-0
MDL number:
MFCD00131282
UNSPSC Code:
12352202
eCl@ss:
42030116
NACRES:
NA.61
Pricing and availability is not currently available.

biological source

human

Quality Level

200

form

lyophilized powder

technique(s)

enzyme immunoassay: suitable

impurities

HIV and hepatitis B antigen, tested negative

solubility

H2O: soluble 20 mg/mL

UniProt accession no.

P02008
P02042
P02100
P09105
P68871
P69891
P69892
P69905

storage temp.

2-8°C

Gene Information

human ... HBA1(3039) , HBA2(3040) , HBB(3043) , HBD(3045) , HBE1(3046) , HBG1(3047) , HBG2(3048) , HBQ1(3049) , HBZ(3050)

General description

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.

Application

Hemoglobin was used in the development of a rapid enzyme immunoassay for the detection of retinol-binding protein.[1] It was also used in the inhibition of human platelet reactivity by endothelium-derived relaxing factor.[2]

Biochem/physiol Actions

The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation. Deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.
Oxygen transporter, NO scavenger

Caution

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chiao-Wang Sun et al.
Circulation, 139(23), 2654-2663 (2019-03-25)
Nitrosation of a conserved cysteine residue at position 93 in the hemoglobin β chain (β93C) to form S-nitroso (SNO) hemoglobin (Hb) is claimed to be essential for export of nitric oxide (NO) bioactivity by the red blood cell (RBC) to
Mieke Steenbeke et al.
Clinical chemistry and laboratory medicine, 58(10), 1759-1767 (2020-07-11)
Objectives In this study, the possibilities of Fourier-transformed infrared spectroscopy (FTIR) for analysis of urine sediments and for detection of bacteria causing urinary tract infections (UTIs) were investigated. Methods Dried urine specimens of control subjects and patients presenting with various
Leila Pishkar et al.
Journal of biomolecular structure & dynamics, 35(3), 603-615 (2016-05-07)
In this study, a novel method to probe molecular interactions and binding of human hemoglobin (Hb) with nanodiamond (ND) was introduced based on the surface tension measurement. This method complements conventional techniques, which are basically done by zeta potential and
Nai-Jia Huang et al.
Nature communications, 8(1), 423-423 (2017-09-06)
A short half-life in the circulation limits the application of therapeutics such as single-domain antibodies (VHHs). We utilize red blood cells to prolong the circulatory half-life of VHHs. Here we present VHHs against botulinum neurotoxin A (BoNT/A) on the surface
Rebeca M Torrente-Rodríguez et al.
PloS one, 12(4), e0175056-e0175056 (2017-04-05)
The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs)
View All Related Papers

Chromatograms

application for HPLC

Questions

1–10 of 11 Questions  

  1. Hi, what is the bulk density of this product ?

    1 answer

    1. Unfortunately, the bulk density of this product is not determined.

      Helpful?

  2. What is the % purity of the lyophilised powder of Hemoglobin Human??

    1 answer

    1. This product is a crude preparation. The purity has not been determined. Unfortunately, purified human hemoglobin is not available at this time.

      Helpful?

  3. Is it LPS-free? when i use this hemoglobin for cell treatment, should I treat polymyxin b B also?

    1 answer

    1. This product is not tested for endotoxin. For use with applications that may be sensitive to presence of LPS, pre-treatment with polymyxin B is a viable approach and is known to effectively neutralize endotoxins. See the link below to review product P1411 Polymyxin B-Agarose, which is recommended for endotoxin removal:
      https://www.sigmaaldrich.com/US/en/product/sigma/p1411

      Polymyxin B-Agarose datasheet with protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/249/698/p1411pis.pdf

      Helpful?

  4. What are the reconstitution instructions for the product, including the amount of liquid and suggested concentration? Additionally, what is the stability of the product after reconstitution?

    1 answer

    1. The product H7379 is tested for solubility at 20 mg/ml during quality control testing. In the past, there was a hemoglobin standard available at 18g/dL or 18% in lyophilized form, mixed with Drabkins reagent to produce a stable cyanomethemoglobin solution. This reagent was stated to be stable for 6 months, with notes indicating that lower concentrations were not necessarily stable. The absorbance values for this solution are typically read at 540 nm.

      After reconstitution, if blood hemoglobin levels are not being determined, it is suggested to take the absorbance in water, scanning the wavelengths from 500-700 nm. A dilution should be performed to achieve an absorbance within the general range of 0.7 to 1.5. After measuring the absorbance of the freshly prepared solution, a baseline is established, and periodic measurements of the absorbance can be taken to determine if the values are trending lower, indicating the need to prepare a fresh solution.

      Helpful?

  5. What is the hemoglobin concentration in the product H7379-1G?

    1 answer

    1. The Specification Sheet for H7379 does not list any purity or concentration. The product is assumed to be 100% hemoglobin. Therefore, if 1 mg of H7379 is dissolved in 1 ml of deionized water, the concentration would be 1 mg/ml.

      Helpful?

  6. How is the human hemoglobin being purified/ desalted after extraction?

    1 answer

    1. Unfortunately the manufacture method is considered proprietary.

      Helpful?

  7. I bought this product in June 2022 and have been using it and storing it properly and was wondering the shelf life of this Product H739-5G

    1 answer

    1. This product does not contain an expiration date, it contains an recommended retest date of roughly 4 years. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. Please view the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/H7379-BULKSLCN4002.pdf

      Helpful?

  8. How many donors are used to make a batch of Product H7379, Hemoglobin human?

    1 answer

    1. This will vary with the batch size, but may come from as many as several hundred donors. All donors are tested and found negative for antibodies to HIV-1/HIV-2, HCV, for HBSAG, and for syphilis.

      Helpful?

  9. Is Product H7379, Hemoglobin human, methemoglobin?

    1 answer

    1. Yes. The iron in hemoglobin will oxidize by exposure to air unless special handling and packaging is used.  Because this product is exposed to air during purification and packaging, it will be predominantly methemoglobin.

      Helpful?

  10. How can Product H7379, Hemoglobin human, be reduced?

    1 answer

    1. A procedure for reduction of methemoglobin may be found in the product sheet.

      Helpful?

1–10 of 11 Questions  

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