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F3040

Monoclonal ANTI-FLAG® M1 antibody

clone M1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD00212941
Conjugate:
unconjugated
Clone:
M1, monoclonal
Application:
WB
Citations:
139
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biological source

(Bioreactor)

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

western blot: 10 μg/mL

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.


Chromatin immunoprecipitation (1 paper)


Immunofluorescence (1 paper)

Learn more product details in our FLAG® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Monoclonal ANTI-FLAG® M1 antibody clone M1, purified immunoglobulin, buffered aqueous solution

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Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Sigma-Aldrich

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Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

CY3 conjugate

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

biological source

(Bioreactor)

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

clone

M1, monoclonal

clone

M5, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

technique(s)

western blot: 10 μg/mL

technique(s)

western blot (chemiluminescent): 10 μg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

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flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
0000512639
0000512639
0000466452
0000466452
0000410739

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Mohammad Al Sorkhy et al.
BMC cancer, 12, 45-45 (2012-01-28)
Spy1 is a novel 'cyclin-like' activator of the G1/S transition capable of enhancing cell proliferation as well as inhibiting apoptosis. Spy1 protein levels are tightly regulated during normal mammary development and forced overexpression in mammary mouse models accelerates mammary tumorigenesis.
Min Luo et al.
Cell research, 20(2), 211-222 (2010-01-27)
As a critical apoptosis executioner, caspase-3 becomes activated and then enters into the nucleus to exert its function. However, the molecular mechanism of this nuclear entry of active caspase-3 is still unknown. In this study, we revealed that caspase-3 harbors
Reem Berro et al.
Journal of virology, 82(14), 7155-7166 (2008-05-16)
The human immunodeficiency virus type 1 (HIV-1) Tat is a 14-kDa viral protein that acts as a potent transactivator by binding to the transactivation-responsive region, a structured RNA element located at the 5' end of all HIV-1 transcripts. Tat transactivates
View All Related Papers

Global Trade Item Number

SKUGTIN
F3040-5MG04061833420614
F3040-.2MG04061833613504
F3040-1MG04061833613511

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