For continuous use, this product should be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/293/677/d9663dat-ms.pdf
D9663
Donkey serum
Synonym(s):
Serum
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Biological source:
donkey
Sterility:
non-sterile
Shipped in:
dry ice
Pricing and availability is not currently available.
biological source
donkey
Quality Segment
sterility
non-sterile
contains
preservative
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
General description
Donkey serum is pooled from a normal donor herd. In immunoassays, normal donkey serum can serve as a blocking agent and a control. It is used as a blocking agent in various studies for immunohistochemistry and immunocytochemistry, ranging from 5% to 20%.[1]
Application
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility - | sterility non-sterile |
| biological source donkey | biological source goat | biological source donkey | biological source rabbit |
| application(s) research pathology | application(s) research pathology | application(s) - | application(s) - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
10 - Combustible liquids
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Can this product be frozen and thawed without affecting the quality?
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where can I find the protocol to reconstitute this donkey serum
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A protocol is not associated with this product. A preservative is added to this serum, making it unsuitable for cell culture. However, in blocking applications, it is typically used at a concentration of 5% - 20%. It may be helpful to view the 'Peer Reviewed Papers' and 'Protocols and Articles' section of the product detail page.
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Is heat inactivation required for D9663 in immunofluorescence (IF)? I found information stating that it is not necessary for immunohistochemistry (IHC). If this applies to IF as well, what is the reason behind it?
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Donkey serum does not require heat inactivation for immunofluorescence. It is important to note that this is not a sterile product. While the lab may have routinely heat inactivated their serums for immunofluorescence, this is likely for worker safety rather than a specific requirement for performing immunofluorescence.
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Does donkey serum D9663-10ML require heat inactivation prior to immunohistochemical assay?
1 answer-
There is no need to heat inactivate donkey serum for blocking purposes.
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