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CY0100

Sigma-Aldrich

Cytochrome c Reductase (NADPH) Assay Kit

1 kit sufficient for 100 tests, determining cytochrome c reductase activity

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

200

usage

 kit sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... POR(5447)

Related Categories

Biochem/physiol Actions

Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.

Features and Benefits

  • Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation
  • Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics
  • Analyze enrichment and function of ER

Analysis Note

The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.

Kit Components Only

Product No.
Description
  • Assay Buffer 100 mL
  • Enzyme Dilution Buffer 25 mL
  • Cytochrome c 50 mg
  • Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
  • Cytochrome c Oxidase Inhibitor Solution 1 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDS

related product

Product No.
Description
Pricing

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H301,H412

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 3

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein and gene structure and regulation of NADPH-Cytochrome P450 oxidoreductase.
Shen, A. L. and Kasper, C. B. et al.
Handbook of Experimental Pharmacology, 105, 35-59 (1993)
Xiyan Li et al.
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Seasonal variations of pollution biomarkers in two populations of Corbicula fluminea (Muller).
Vidal, M.-L., et al.
Comparative Biochemistry and Physiology, 131, Part C, 133-151 (2002)
Camila Ritter et al.
Molecular neurobiology, 57(5), 2420-2435 (2020-02-26)
Central neuropathic pain is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients' daily activities. The neuroinflammation process and mitochondrial dysfunction in the PMS lesions generate reactive species.
A Hussain et al.
Human & experimental toxicology, 39(10), 1318-1332 (2020-04-30)
Various studies in rodents have shown that nanoparticles are transferred to the breast milk. Under the present study, lactating Wistar rats were repetitively gavaged 5, 25, and 50 mg/kg bw of zinc oxide nanoparticles (ZnO-NPs) and 50 mg kg-1 bw
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