α-Chymotrysin Protease

Name: α-Chymotrysin Protease
Brand: SIGMA

Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas

Select a Size

Change View

About This Item

CAS Number:

9004-07-3

UNSPSC Code:

12352204

eCl@ss:

42010112

NACRES:

NA.26

EC Number:

232-671-2

MDL number:

MFCD00130481

EC Number:

3.4.21.1 (BRENDA, IUBMB)

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

α-Chymotrypsin from bovine pancreas, suitable for protein sequencing, salt-free, lyophilized powder

grade

Proteomics Grade

Quality Segment

200

form

salt-free, lyophilized powder

mol wt

25 kDa

suitability

suitable for protein sequencing

UniProt accession no.

P00767

storage temp.

2-8°C

Gene Information

cow ... CTRB1(618826)

Description

General description

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Application

α-Chymotrypsin from bovine pancreas is used for the following applications:

Protein Identification by mass spectrometry (MS)
The isolation and characterization of myosin heavy chains
Toxin preparation
The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu²⁺ and Hg.